Part:BBa_K1993035
IRES-Luciferase-T2A-dtomato-T2A-hFTH
In order to assemble our device easily since we had constructed plasmids by Gateway technology, we constructed a composite part transcripting IRES and Luciferase-T2A-dTomato-T2A-eGFP which is convenient for running Gateway BP and LR reactions.
We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translating initiation in an end-independent manner, as part of the greater process of protein synthesis.
Firefly(Photinus pyralis) Luciferase (Details can be seen from BBa_K1993018) is a kind of oxidative enzyme that produce bioluminescence. It is able to oxidize luciferin and produce detectable bioluminescence. It’s convenient to observe biological processes, especially allowing for observation of cells non-invasively. So we added such a gene to ensure observation in vivo.
To expand the function of our composite part, we added two more genes: dTomato(Details can be seen from BBa_K1993020) used for observation in vitro conveniently and easily; hFTH(Details can be seen from BBa_K1993021), another protein that ccould be observed in vivo by MRI and applied to human body. What’s more, in order to make sure the expression of all three proteins, we added a T2A gene(Details can be seen from BBa_K1993019) between every two protein coding sequences. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1396
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